BBa_K563054

BBa_K563054 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K563054

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Guo Jing

Date created: 2011-10-04 11:00:00

Date modified: 2015-05-08 01:12:42

Vector pYD, and can transfer into nucles of S.cerevisivae

Details

• Types: DnaRegion
• Roles: Plasmid_Backbone; plasmid_vector
• Sequences: BBa_K563054_sequence (Version 1)

Description

pYD is a 5.0kb expression vector designed for expression, secretion, and display of proteins on the extracellular surface of S.cerevisivae cells. Features of this vector allow regulated expression, secretion and detection of expressed proteins.

Notes

In our project, to make our mutation of Tor2 easier, we separate complete Tor2 into three parts and mutate them independently. So we reconstruct pYD vector to add certain restriction sites for linking mutant Tor2 and original Tor2

Source

AGA2, GAL1, TRP5 and CEN come from S.cerevisivae. Amppicillin resistance gene and the pUC origin for selection and replication come from E.coli

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
GAL1	1,451	promoter, feature/promoter
T7 promoter	475,494	promoter, feature/promoter
Amppicillin	2931,3791	feature/cds, CDS

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K563054/1

Member of these Collections

• iGEM Parts Registry
• igem_plasmid_vectors