Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: You Wang
Date created: 2011-09-28 11:00:00
Date modified: 2015-05-08 01:12:43
PT7-metGM
| Types | DnaRegion |
| Roles | Translational_Unit engineered_region |
| Sequences | BBa_K567014_sequence (Version 1) |
Description
T7 promoter-metG(mutated). This biobrick is constructed by putting the mutated metG (Met-RS) under the control of T7 promoter and lac operator. We have cloned metG from E.coli and have used error-prone PCR to amplify the metG. Kana gene with start codon substituted for CGA is used to testify the function of mutated metG. When this biobrick and metY-CGA (BBa_K567016) are co-transformed into the cell, the cells can survive on the LB Kana plate.Notes
Overlapping PCR is usedSource
metG from E.coli. Kana gene derived from pET28a| Sequence Annotation | Location | Component / Role(s) |
| T7 promoter lac operator rbs MetRS(mutated) T7 TE | 2,18 21,44 75,81 89,2122 2209,2256 | promoter feature/promoter operator feature/operator feature/rbs ribosome_entry_site feature/cds CDS feature/stem_loop stem_loop |