BBa_K571001

BBa_K571001 Version 1

Component

This part has been discontinued.

Please consider using BBa_K571003 as a replacement.

Source: http://parts.igem.org/Part:BBa_K571001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Lu-Chu Ke

Date created: 2011-10-02 11:00:00

Date modified: 2015-05-08 01:12:44

acsCD/pSB1C3

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K571001_sequence (Version 1)

Description

This biobrick contains the remaining 2 cellulose synthase enzyme complex, acsC and acsD of 4,363 bp in total (full CDS), again containing Xba I and Spe I restriction sites cloned in pSB1C3. The acsC gene is the main composite in the formation of the membrane complex of cellulose synthase and proposed to be involved in the export of the polymer across the bacterial cell wall.
The acsD gene is involved in the crystallization of the mature cellulose by cleaves the intrastrand ??-1,4 linkages in the cellulose chain and is proposed to have a role in the release of the growing polymer from the cell.

Notes

acsCD is long base pairs gene, so more than one primer is used during PCR. It is also advisable to separate PCR into a few times to get the most satisfied results.

Source

*organism: Gluconacetobacter hansenii ATCC 23769
*GenBank: AB091060.1

Other Properties

• igem#experience: None
• igem#sampleStatus: Discontinued
• igem#status: Deleted
• isReplacedBy: BBa_K571003/1
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K571001/1

Member of these Collections

• iGEM Parts Registry