Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lu-Chu Ke
Date created: 2011-10-02 11:00:00
Date modified: 2015-05-08 01:12:44
R0011+acsAB/pSB1C3
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K571005_sequence (Version 1) |
Description
The biobrick is composite element, combination of BBa_K571000 and BBa_K571003. The promoter binding to the upstream of acsAB gene, IPTG as the inducer, activates the acsAB gene, producing Cyclic-di-GMP binding domain and UDP-forming domain.The sequence of acsAB and promoter is amplified by PCR, followed by enzyme digestion and ligation to bind the 2 sequences with the backbone-pSB1C3. The order of restriction enzyme is EcoRI, XbaI and SpeI.
Notes
It is a very long sequence, therefore below is the considerations to take note during cloning.*The concentration agarose gel during gel electrophoresis should be about 0.8-1%
*The enzyme digestion reaction time is advised to be elongated so that digestion could be more perfect.
*The respective restriction enzyme at the both end of primer should not cut the sequence of another gene
Source
*acsAB: GenBank AB091060.1*promoter: iGEM registry of standard biological parts
| Access | Instance | Definition |
| public public | BBa_K571004 BBa_K571000 | BBa_K571004 BBa_K571000 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K571004 BBa_K571000 | 1,80 87,4739 | BBa_K571004 BBa_K571000 |