BBa_K577884

BBa_K577884 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K577884

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Alberto Purwada

Date created: 2011-09-10 11:00:00

Date modified: 2015-05-08 01:12:46

pC+AraC+pBad+YFPc

Details

• Types: DnaRegion
• Roles: Reporter; engineered_region
• Sequences: BBa_K577884_sequence (Version 1)

Description

This reporter part is a combination of a constitutive promoter, AraC gene, a pBad promoter (inducible with arabinose), and a YFP gene. The purpose of this part is to provide the user with a simple, but complete, inducible YFP genetic device. This is useful if the user plans to build a larger plasmid construct in a short time or characterize a specific part. This part can be inserted in a cell line that does not contain any AraC in its genome, which is important if the user is not using Top 10 cells.

Notes

There is no specific design consideration required in the making of this part.

Source

This part is obtained from ligating several biobricks.

Components

Access	Instance	Definition
public	BBa_I13458	BBa_I13458
public	BBa_I13453	BBa_I13453
public	BBa_E0430	BBa_E0430

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I13458	1,1114	BBa_I13458
BBa_I13453	1123,1252	BBa_I13453
BBa_E0430	1261,2138	BBa_E0430

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K577884/1

Member of these Collections

• iGEM Parts Registry