BBa_K577889

BBa_K577889 Version 1

Component

This part has been discontinued.

Please consider using BBa_K206004 as a replacement.

Source: http://parts.igem.org/Part:BBa_K577889

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Alberto Purwada

Date created: 2011-09-10 11:00:00

Date modified: 2015-05-08 01:12:46

pBad+RFPc

Details

• Types: DnaRegion
• Roles: engineered_region; Reporter
• Sequences: BBa_K577889_sequence (Version 1)

Description

This reporter part is a combination of a pBad promoter (inducible with arabinose) and a RFP gene. The purpose of this part is to provide the user with a simple, but complete, inducible genetic device. This is useful if the user plans to build a larger plasmid construct in a short time or characterize a specific part. But It is important for the user to determine if his or her cell line contain AraC gene, since AraC gene is required to actually induce pBad promoter with arabinose. See BBa_K577882 or BBa_I13458 for more information.

Notes

The RBS, RFP, and Terminator were taken from the BBa_J61002 backbone using SpeI and PstI restriction enzymes.

Source

This part comes from ligating 2 biobricks.

Components

Access	Instance	Definition
public	BBa_I13453	BBa_I13453
public	BBa_B0034	BBa_B0034
public	BBa_E1010	mRFP1
public	BBa_B0015	BBa_B0015

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I13453	1,130	BBa_I13453
BBa_B0034	139,150	BBa_B0034
BBa_E1010	157,837	mRFP1
BBa_B0015	871,999	BBa_B0015

Other Properties

• igem#experience: None
• igem#partStatus: Released HQ 2013
• igem#sampleStatus: Discontinued
• igem#status: Deleted
• isReplacedBy: BBa_K206004/1
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K577889/1

Member of these Collections

• iGEM Parts Registry