Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Mitesh Agrawal
Date created: 2011-09-27 11:00:00
Date modified: 2015-05-08 01:12:46
Cas 9 + Repeat/Spacer Region
| Types | DnaRegion |
| Roles | sequence_feature DNA |
| Sequences | BBa_K582003_sequence (Version 1) |
Description
The Spacer/Repeat Region part has been extracted from the Streptococcus Thermophilus LMD9 genome sequence. If inserted along with the CRISPR protein system and sigma factor 70, we hypothesize that the spacer/repeat region should express itself and make bacteria resistant to which the spacer region is homologous.Notes
The sequence has the restriction sites present on the pSB1C3 backbone. We will have to subject it to mutagenesis to make the biobrick functional.Source
http://www.ncbi.nlm.nih.gov/nuccore/116626972?report=graph&from=1379975&to=1384141 for Cas9http://www.ncbi.nlm.nih.gov/nuccore/116626972?report=genbank&from=648900&to=650325 for Repeat/Spacer Region
| Access | Instance | Definition |
| public public | BBa_K582001 BBa_K582002 | BBa_K582001 BBa_K582002 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K582001 BBa_K582002 | 1,4167 4176,5601 | BBa_K582001 BBa_K582002 |