BBa_K590010

BBa_K590010 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K590010
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Michael Brasino, Alicia Wong, Rashmi Ravichandran
Date created: 2011-09-14 11:00:00
Date modified: 2015-05-08 01:12:47

pGA1A3, Gibson assembly plasmid (bglBrick) with pLac-GFP insert



Types
DnaRegion

Roles
Plasmid_Backbone

plasmid_vector

Sequences BBa_K590010_sequence (Version 1)

Description

Backbone that is Gibson reaction friendly (Bgl Brick)

Notes

sequence confirmed

Source

Made from Gibson reaction

Sequence Annotation Location Component / Role(s)
pGA Suffix
E. coli his operon terminator
terminator stem loop
Vr
rep(pMB1)
ORI
ampR
Vf2
end of terminator
terminator stem loop
pGA Prefix
1,21
22,93
30,64
157,176
261,875
276,276
1035,1895
2018,2037
2094,2097
2107,2126
2135,2156
engineered_region feature/BioBrick
feature/misc sequence_feature
stem_loop feature/stem_loop
primer_binding_site feature/primer_binding
CDS feature/cds
feature/misc sequence_feature
CDS feature/cds
feature/primer_binding primer_binding_site
sequence_feature feature/misc
stem_loop feature/stem_loop
feature/BioBrick engineered_region
igem#experience
Works
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K590010/1