BBa_K608101

BBa_K608101 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K608101

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Julia M??ller

Date created: 2011-09-13 11:00:00

Date modified: 2015-05-08 01:12:52

CcaR, green light response regulator

Details

• Types: DnaRegion
• Roles: Signalling; engineered_region
• Sequences: BBa_K608101_sequence (Version 1)

Description

CcaR is the response regulator to the green light receptor CcaS.
It belongs to the family of OmpR regulator class. CcaR consists of an N-terminal receiver domain that can be phosphorylated by CcaS and a C-terminal DNA-binding domain that binds directly to the promoter region of cpcG2.

Notes

We deleted an EcoRI restriction site in CcaR, in order to make it compatible to the iGEM standart.

Source

It was PCR-amplified from the genomic sequence of Synechocystis sp. PCC 6803.
The following primers were used:
XbaI_RBS_ccaR_up, TATTCTAGATTCACACAGGAAACCTCACTAATGAGAATTCTTTTAGTGGAGGATG
ccaR_SpeI_PstI_dw,GCCGGACTGCAGCGGCCGCTACTAGTCTAGTTTTTCCCTTGGCACAAAG

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K608101/1

Member of these Collections

• iGEM Parts Registry