Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: R??diger Trojok
Date created: 2011-09-13 11:00:00
Date modified: 2015-05-08 01:12:52
Precipitator
| Types | DnaRegion |
| Roles | Protein_Domain polypeptide_domain |
| Sequences | BBa_K608406_sequence (Version 1) |
Description
Protein domain of the Precipitator. It is an new Leucine Rich Repeat(LRR) with C and N-terminal hagfish domain fragments capping the artifical middle part. This part is one version of three different, designed to bind Nickel by Histidines which are grouped together pointing away from the horseshoe shaped protein. The Precipitator versions differ only in the pattern the position of the Histidine groups. Compare our 3D models on our wiki.This protein can be used to complex up to 4 Nickel or Cobalt. However the principal design oft he protein is of a particular interest, too. LRR are highly conserved motifs throughout evolution. They appear in all kingdoms of life in almost every thinkable role (Ligases, Receptors, Toxins etc.). Their core is highly conserved and provides a very stable backbone, while the intermediate, non-conserved aminoacids are almost freely interchangeable.
We only submitted one of the three versions, to reduce redundancy in the registry. Please contact us for any questions.
Notes
Here we investigated an optimal set of non-conserved aminoacids by analysing large sets of similar proteins and databases. You can use this piece of work as a template to design your own protein and give it any function you like, by simply interchanging aminoacids and fusing other domains on the N or C termini.To guarantee proper folding and to shield off the hydrophobic core, a well studied fragment of an LRR protein coming from hagfish was used. This technique was investigated before
Source
The part is constructed from the following protein sequences(PDB: 2z66) N-Terminal hagfish
(PDB: 2z62) C-Terminal hagfish
(PDB: 3cvr) bacterial ligase
| Sequence Annotation | Location | Component / Role(s) |
| Precipitator N-Terminal Hagfish PDB:2z66 C-Terminal Hagfish PDB:2z62 | 1,763 1,57 687,763 | CDS feature/protein feature/conserved conserved_region feature/misc sequence_feature |