BBa_K611008

BBa_K611008 Version 1

Component

This part has been discontinued.

Please consider using BBa_R0040 as a replacement.

Source: http://parts.igem.org/Part:BBa_K611008

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Nicholas Csicsery

Date created: 2011-09-17 11:00:00

Date modified: 2015-05-08 01:12:53

R0040+B0034+E0040+B0015+I13453+B0034

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K611008_sequence (Version 1)

Description

This part is used to characterize mutants of BBa_C0040 (TetR). By adding a mutant of BBa_C0040 after this part, one can measure several of its properties through its regulation of GFP expression. Concentration of the added TetR mutant can be varied through different arabinose concentrations as long as AraC is present.

Notes

There is no terminator at the end, so the part needs to be used in a plasmid that has a terminator after the BioBrick suffix (such as pSB1A3 or pSB1C3).

Source

Built from existing parts in the registry though BioBrick assembly.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0040	1,54	feature/BioBrick, engineered_region
TetR 1	1,19	non_covalent_binding_site, feature/binding
-35	20,25	feature/promoter, promoter
TetR 2	26,44	non_covalent_binding_site, feature/binding
-10	43,48	feature/promoter, promoter

Other Properties

• igem#experience: None
• igem#partStatus: Released HQ 2013
• igem#sampleStatus: Discontinued
• igem#status: Deleted
• isReplacedBy: BBa_R0040/1
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K611008/1

Member of these Collections

• iGEM Parts Registry