BBa_K611010

BBa_K611010 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K611010
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nicholas Csicsery
Date created: 2011-09-17 11:00:00
Date modified: 2015-05-08 01:12:53

LacI Repressor Characterization Construct



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K611010_sequence (Version 1)

Description

This part is used to characterize mutants of BBa_C0012 (LacI). By adding a mutant of BBa_C0012 after this part, one can measure several of its properties through its regulation of GFP expression. Concentration of the added TetR mutant can be varied through different arabinose concentrations as long as AraC is present.

Notes

There is no terminator at the end, so the part needs to be used in a plasmid that has a terminator after the BioBrick suffix (such as pSB1A3 or pSB1C3).

Source

Built from existing parts in the registry though BioBrick assembly.

igem#experience
None
 
igem#partStatus
Released HQ 2013
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/myers
 
synbiohub#ownedBy
user/james
 
synbiohub#topLevel
BBa_K611010/1