BBa_K613003

BBa_K613003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K613003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Alessandro Ferrari, Vincent Zimmern, Nadine Guenat, Henrike Niederholtmeyer

Date created: 2011-09-19 11:00:00

Date modified: 2015-05-08 01:12:54

T7 promoter family member

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K613003_sequence (Version 1)

Description

Parts K613001 through K613006 are T7 promoter variants. K613001 is the T7 promoter wildtype. The other sequences were derived by single base pair mutations that have been shown to alter its strength. The part includes a strong ribosomal binding site. Its strength was determined by cloning it in front of mRFP1 reporter and measuring fluorescence of BL21 cells containing a low copy number plasmid containing the reporter construct.

Notes

Imburgio, D., Rong, M., Ma, K. & McAllister, W.T. Studies of promoter recognition and start site selection by T7 RNA polymerase using a comprehensive collection of promoter variants. Biochemistry 39, 10419???10430 (2000).

Source

synthetic

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
RBS	96,101	ribosome_entry_site, feature/rbs
T7 promoter	30,52	feature/promoter, promoter

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K613003/1

Member of these Collections

• iGEM Parts Registry