BBa_K622000

BBa_K622000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K622000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Fumitaka Hashiya
Date created: 2011-09-30 11:00:00
Date modified: 2015-05-08 01:12:55

rpoN: coding sigma 54 factor



    • Details

    Types
    DnaRegion

    Roles
    engineered_region

    Translational_Unit

    Sequences BBa_K622000_sequence (Version 1)

    Description

    This part codes sigma 54 factor of E.coli and its RBS.
    We use this part to make nutritional switch which is activated under poor availability of combined nitrogen.
    The complex of sigma 54 factor and the other transcription factor activates sigma 54 promoter.

    Notes

    Original CDS of rpoN has one restriction site of Pstl.
    We removed it by iPCR.

    Source

    Genomic DNA of E.coli

    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K622000/1
     

    Attachments

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