Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Glasgow Uni 2011
Date created: 2011-09-15 11:00:00
Date modified: 2015-05-08 01:13:03
LOV2 Domain
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_K660000_sequence (Version 1) |
Description
The LOV (Light-Oxygen-Voltage) domain is a photoreceptor that responds to blue light. In nature it was first found to be involved in the phototropism response in plants and has since been found to be present in fungi and bacteria also. It has been shown to be coupled to many domains, for example phosphodiesterase or kinases.We are using it as a reporter due to its ability to function in anoxic conditions. This is particularly useful in biofilms and is a function that fluorescent proteins derived from GFP do not have.
Notes
Illegal restriction sites were found so Site Directed Mutagenesis has to be performed.Source
The LOV domain was originally isolated from Arabidopsis and has been codon optimised for expression in E.coli.| Sequence Annotation | Location | Component / Role(s) |
| LOV2 domain | 24,359 | CDS feature/protein |