BBa_K660616

BBa_K660616 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K660616

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Ellin-Kristina Hillert

Date created: 2011-09-21 11:00:00

Date modified: 2015-05-08 01:13:03

pBAD with strong RBS

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_K660616_sequence (Version 1)

Description

This regulatory part is a composite of a strong pBAD promoter (Part:BBa_K206000) and a strong ribosome binding site (Part:BBa_B0034), for use in selective expression of any device.
Using this composite will simplify the making of constructs, as it reduces the number of ligations necessary to produce a full pBAD controlled construct.

Notes

This composite part was made using the restriction enzyme AlwNI instead using the standard biobrick assembly method.

Source

Both components of the composite part were obtained from the Registry of Standard Biological Parts

Components

Access	Instance	Definition
public	BBa_K206000	pBAD
public	BBa_B0034	BBa_B0034

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K206000	1,130	pBAD
BBa_B0034	139,150	BBa_B0034

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K660616/1

Member of these Collections

• iGEM Parts Registry