Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Silvia J Ca??as
Date created: 2011-10-27 11:00:00
Date modified: 2015-05-08 01:13:03
Allows the measurement of the CBP promoter activity
| Types | DnaRegion |
| Roles | Measurement engineered_region |
| Sequences | BBa_K669006_sequence (Version 1) |
Description
Chitooligosaccharide-binding protein (CBP) is a cytoplasmatic protein that regulates the transcriptional activity of the ChiS sensor.When chitin is present in the external media, CBP binds with GlcNAc oligomers (chitin degradation products) and liberates the sensor for the up-regulation of the chitinolitic degradation cascade in Vibrio fischeri ES114.
Notes
mCherry protein is used from a BioBrickSource
Vibrio fischeri| Sequence Annotation | Location | Component / Role(s) |
| BBa_K669006 BBa_B0034 BBa_J06702 conserved BBa_J06504 mCherry BBa_J06702 BBa_B0034 BBa_B0034 BBa_J06702 conserved conserved BBa_J06504 mCherry mCherry BBa_J06504 C->T (removing PstI site) C->T (removing PstI site) C->T (removing PstI site) BBa_B0010 stem_loop BBa_B0012 T7 TE polya stop BBa_B0010 BBa_B0010 stem_loop stem_loop BBa_B0012 BBa_B0012 T7 TE T7 TE polya polya stop stop BBa_B0034 BBa_J06702 BBa_B0034 BBa_J06702 conserved conserved BBa_J06504 mCherry BBa_J06504 mCherry C->T (removing PstI site) C->T (removing PstI site) BBa_B0010 BBa_B0010 stem_loop stem_loop BBa_B0012 BBa_B0012 T7 TE T7 TE polya polya stop stop BBa_B0034 conserved mCherry BBa_J06504 C->T (removing PstI site) BBa_B0010 stem_loop BBa_B0012 T7 TE polya stop BBa_J06702 | 1,275 1,12 1,869 5,8 19,732 19,729 284,1152 284,295 284,295 284,1152 288,291 288,291 302,1015 302,1012 302,1012 302,1015 370,370 653,653 653,653 741,820 752,795 829,869 836,855 856,869 862,862 1024,1103 1024,1103 1035,1078 1035,1078 1112,1152 1112,1152 1119,1138 1119,1138 1139,1152 1139,1152 1145,1145 1145,1145 1161,1172 1161,2029 1161,1172 1161,2029 1165,1168 1165,1168 1179,1892 1179,1889 1179,1892 1179,1889 1530,1530 1530,1530 1901,1980 1901,1980 1912,1955 1912,1955 1989,2029 1989,2029 1996,2015 1996,2015 2016,2029 2016,2029 2022,2022 2022,2022 2038,2049 2042,2045 2056,2766 2056,2769 2407,2407 2778,2857 2789,2832 2866,2906 2873,2892 2893,2906 2899,2899 2038,2906 | feature/BioBrick engineered_region feature/BioBrick engineered_region feature/BioBrick engineered_region feature/conserved conserved_region engineered_region feature/BioBrick CDS feature/cds engineered_region feature/BioBrick engineered_region feature/BioBrick engineered_region feature/BioBrick engineered_region feature/BioBrick feature/conserved conserved_region feature/conserved conserved_region engineered_region feature/BioBrick feature/cds CDS feature/cds CDS feature/BioBrick engineered_region feature/mutation sequence_alteration feature/mutation sequence_alteration feature/mutation sequence_alteration feature/BioBrick engineered_region stem_loop feature/stem_loop engineered_region feature/BioBrick feature/stem_loop stem_loop feature/polya polyA_site feature/stop stop_codon engineered_region feature/BioBrick engineered_region feature/BioBrick stem_loop feature/stem_loop stem_loop feature/stem_loop feature/BioBrick engineered_region engineered_region feature/BioBrick stem_loop feature/stem_loop feature/stem_loop stem_loop feature/polya polyA_site feature/polya polyA_site feature/stop stop_codon feature/stop stop_codon feature/BioBrick engineered_region feature/BioBrick engineered_region feature/BioBrick engineered_region engineered_region feature/BioBrick conserved_region feature/conserved feature/conserved conserved_region engineered_region feature/BioBrick feature/cds CDS feature/BioBrick engineered_region CDS feature/cds feature/mutation sequence_alteration sequence_alteration feature/mutation feature/BioBrick engineered_region engineered_region feature/BioBrick stem_loop feature/stem_loop feature/stem_loop stem_loop feature/BioBrick engineered_region feature/BioBrick engineered_region feature/stem_loop stem_loop stem_loop feature/stem_loop feature/polya polyA_site feature/polya polyA_site feature/stop stop_codon stop_codon feature/stop engineered_region feature/BioBrick feature/conserved conserved_region CDS feature/cds engineered_region feature/BioBrick feature/mutation sequence_alteration engineered_region feature/BioBrick stem_loop feature/stem_loop engineered_region feature/BioBrick stem_loop feature/stem_loop feature/polya polyA_site stop_codon feature/stop engineered_region feature/BioBrick |