Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Bernadette Miramontes
Date created: 2011-06-22 11:00:00
Date modified: 2015-05-08 01:13:03
Arsenic Detector With Wintergreen
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K675004_sequence (Version 1) |
Description
Promoter arsRp, BBa_K190015 is associated with the dimer of ArsR for the arsenic induced transcription of genes involved in arsenic efflux (arsR, arsB and arsC, which is present on the genome of Escherichia coli str. K-12 substrain MG1655). The sequence shows the typical -10 and -35 region of the promoter. A second region, located at -41.5 from the transcription start site, is thought to bind dimeric ArsR. Upon binding of arsenic, the dimer dissociates and allows the RNA polymerase space to attach itself. The arsenic promoter has been ligated to the wintergreen reporter, BBa_J45119. In the presents of arsenic the device produces wintergreen smell. BBa_J45119 takes as input a transcriptional signal (PoPS) and produce as output the BMST1 enzyme that catalyzes production of methyl salicylate from salicylic acid. Methyl salicylate has a wintergreen smell.Notes
noneSource
none| Access | Instance | Definition |
| public public | BBa_K190015 BBa_J45119 | pArsR BSMT1 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K190015 BBa_J45119 | 1,72 81,1310 | pArsR BSMT1 |