BBa_K676777

BBa_K676777 Version 1

Component

This part has been discontinued.

Please consider using BBa_K185030 as a replacement.

Source:
http://parts.igem.org/Part:BBa_K676777
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kheng Tee Ng
Date created: 2011-09-16 11:00:00
Date modified: 2015-05-08 01:13:04

GyrA cassette



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K676777_sequence (Version 1)

Description

This expression cassette is regulated by an IPTG inducible promoter and will be expressing Gyr A subunits . Gyr A subunit will bind to the DNA ( gyrase binding site) and is responsible for catalysing DNA breakage and resealing in order to change the level of supercoiling in DNA. However in order to introduce negative supercoiling into the DNA , a functional tetrameric gyrase structure with two GyrA and two GyrB should form with the presence of divalent magnesium ions and ATP.

Notes

The wild type Gyr A ORF has a Pst 1 restriction site at the 1212th bp position so site directed mutagenesis is carried out to remove the restriction site.

Source

Gyr A gene is obtained from E.coli genome.

igem#experience
None
 
igem#sampleStatus
Discontinued
igem#status
Deleted
 
isReplacedBy
BBa_K185030/1
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K676777/1