mRFP1

BBa_K678031 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K678031
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: DTU-Denmark-2
Date created: 2011-09-08 11:00:00
Date modified: 2015-05-08 01:13:04

mRFP1-GOI



Types
DnaRegion

Roles
engineered_region

Reporter

Sequences BBa_K678031_sequence (Version 1)

Description

Monomeric red fluorescent protein (mRFP1) is a derivative of the red fluorescent protein cloned from Discosoma coral (DsRed) (1). The successful use of mRFP1 in Aspergillus nidulans has previously been described (2). This part does not contain a stop codon.


{| border="1"
!colspan="8"|Spectroscopic data (1)
|-
|Excitation maximum
|Emission maximum
|Extinction coefficient per chain
|Fluorescence quantum yield
|pKa
|t0.5 for maturation at 37??C
|colspan="2"|Rate of photobleach relative to EGFP
|-
|584 nm
|607 nm
|44,000 M-1cm-1
|0.25
|4.5
|<1h
|fast
7.2
|slow
0.16
|}

Notes

This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains tails at the 5' and 3' ends as shown on the figure below. Please notice that mRFP1-module is not the same as BBa_K678030 or BBa_K678032.

Source

From p71-mRFP1 of the Center for Microbial Biotechnology plasmid collection at the Technical University of Denmark

Sequence Annotation Location Component / Role(s)
Linker 2
RFP
1,8
9,683
feature/misc sequence_feature
feature/cds CDS
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K678031/1