Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jason Fontana, Daniele Rossetto
Date created: 2012-08-20 11:00:00
Date modified: 2015-05-08 01:13:06
Inducible araC-pBAD promoter regulating a sfGFP-tagged M256I CysE
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K731040_sequence (Version 1) |
Description
araC-pBAD regulating CysE M256 (K731030) was fused at his 3' with sfGFP (spaced by a GSGSGS linker) using Gibson's Assembly.Notes
Primers for Gibson's Assembly were designed to add a linker before sfGFP and remove the stop codons from CysE M256I.Source
K731030| Sequence Annotation | Location | Component / Role(s) |
| araC BBa_K731201 PC pBAD RBS BBa_K731010 Spacer Start Linker BBa_K731030 sfGFP Stop Codons | 1,1080 1,1203 1043,1114 1080,1203 1212,1217 1212,2044 1218,1225 1226,1228 2045,2062 1,2058 2063,2782 2776,2782 | CDS feature/protein feature/BioBrick engineered_region feature/promoter promoter feature/promoter promoter ribosome_entry_site feature/rbs feature/BioBrick engineered_region sequence_feature feature/misc start_codon feature/start feature/misc sequence_feature feature/BioBrick engineered_region feature/tag tag feature/stop stop_codon |