BBa_K731400

BBa_K731400 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K731400

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Andrea Tassinari

Date created: 2012-08-20 11:00:00

Date modified: 2015-05-08 01:13:06

IPTG inducible Cysteine desulfhydrase (CysDes)

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K731400_sequence (Version 1)

Description

This composite part was designed to construct a working CysDes gene under the control of an inducible promoter: the presence of IPTG inhibits the LacI repressor protein binding to the LacO operator; in this way it allows the RNApol to bind to the Ptac promoter and trascribe the gene of interest.
In this case the gene of interest is a Cysteine Desulfhydrase, an aminotransferase that converts cysteine into pyruvate, ammonia and hydrogen sulfide.

Notes

LacI+LacIq promoter is an autonomous part (K731300) also designed by our team.

Source

LacI+LacIq promoter comes from pET-21b plasmid.
CysDes gene comes from the genome of Treponema denticola.

Components

Access	Instance	Definition
public	BBa_K731300	BBa_K731300
public	BBa_K731600	BBa_K731600

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K731300	1,1153	BBa_K731300
BBa_K731600	1162,2466	BBa_K731600

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K731400/1

Member of these Collections

• iGEM 2018 Distribution Plate 6
• iGEM 2018 Distribution Plate 6 Well 4K
• iGEM 2018 Part Library
• iGEM 2018 Plasmid pSB1C3
• iGEM 2019 Distribution Plate 4
• iGEM 2019 Distribution Plate 4 Well 4K
• iGEM 2019 Part Library
• iGEM 2019 Plasmid pSB1C3
• iGEM Parts Registry