Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wenjie Wu and Peiran Zhang
Date created: 2012-09-17 11:00:00
Date modified: 2015-05-08 01:13:07
Spot42 generator controlled by aTc
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K737036_sequence (Version 1) |
Description
[J23106,P0440]is a TetR generator, which represses tet promotor and can be induced by aTc.aTc concentration gradient results in different sRNA Spot42 levels in cells, which can be monitored by our galK::GFP generator,where galK is the RBS of GFP and the target for Spot42.
*/pictures stands for high level,leakage level,no Spot42 cases on J61002 vector,when combined with galK::GFP generator in progressing/*
ClaI site and SalI site are well-selected restriction sites,they are used to assembly the artificial region to the 5??? end of Spot42,are compatible for all of the Ampr and Chlr vectors(no unexpected restction sites),whereas all the Kanar vectors will be restricted into fragments when treated with SalI/ClaI.
Notes
ClaI regcognizes ATCGAT,which can be blocked by GATC methylation,pay extra attention to this when you assembly artificial region to Spot42.Source
No| Access | Instance | Definition |
| public public public public | BBa_J23106 BBa_P0440 BBa_R0040 BBa_K737058 | BBa_J23106 BBa_P0440 p(tetR) BBa_K737058 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23106 BBa_P0440 BBa_R0040 BBa_K737058 | 1,35 44,883 892,945 954,1062 | BBa_J23106 BBa_P0440 p(tetR) BBa_K737058 |