psb1C3_int

BBa_K743006 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K743006

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Date created: 2012-09-19 11:00:00

Date modified: 2015-05-08 01:13:09

psb1C3_IntK recombination plasmid for Synechocystis PCC6803

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K743006_sequence (Version 1)

Description

This plasmid allows the integration of a Kanamycin resistance casette followed by a double terminator in to Synechocystis chromosome, this allows for selection in this cyanobacteria. It has a Chloramphenicol resistance casette for selection in E.coli
The use of BBa_K743000 and BBa_K743001 as recombination sites has no phenotypic effects on the cells.
The plasmid is intended to be used as a starting backbone for further adition of dna parts by Gibson assembly.
It was used by UC_Chile 2012 team to succesfully transform Synechocystis http://2012.igem.org/Team:UC_Chile

Source

RS1 and RS2 were obtained from Synechocystis genomic DNA, both the kanamycin resistance (BBa_P1003) and the double terminator (BBa_B0015) are from the registry of standard biological parts.

Components

Access	Instance	Definition
public	BBa_S05070	BBa_S05070
public	BBa_S05069	BBa_S05069

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_S05070	1,1497	BBa_S05070
BBa_S05069	1506,2329	BBa_S05069

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K743006/1

Member of these Collections

• iGEM Parts Registry