BBa_K743014

BBa_K743014 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K743014

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Simon Alamos

Date created: 2012-09-21 11:00:00

Date modified: 2015-05-08 01:13:09

after dusk -> LuxAB luciferase (Photorabdus luminiscens)

Details

• Types: DnaRegion
• Roles: Generator; engineered_region
• Sequences: BBa_K743014_sequence (Version 1)

Description

This part is designed to express the LuxA and LuxB genes from Photorabdus luminiscens in a circadian manner, peaking a 2 hours after subjective dusk (or hour 14 of circadian time if you prefer).
When transfromed into Syenchocystis PCC6803, the endogenous circadian transcriptional mechanism controls the activity of the Pta promoter making the heterodimeric LuxAB luciferase to be expressed at the specified time.

This permits for the emition of light by the luciferase reaction to be restricted to this time of the day (dependent on the addition or endogenous production of substrates)

Source

LuxAB coding sequence was amplified from http://partsregistry.org/Part:BBa_K216008.
Pta promoter is from BBa_K743003

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K743003 Pta promoter	1,150	feature/promoter, promoter
putative rbs (not known)	120,150	feature/rbs, ribosome_entry_site
LuxA	151,1230	feature/protein, CDS
rbs	1231,1247	ribosome_entry_site, feature/rbs
LuxB	1248,2228	feature/protein, CDS
stop codons	2229,2234	feature/tag, tag
BBa_K216008	151,2234	engineered_region, feature/BioBrick

Other Properties

• igem#experience: Fails
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K743014/1

Member of these Collections

• iGEM Parts Registry