Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Bernardo Pollak
Date created: 2012-09-21 11:00:00
Date modified: 2015-05-08 01:13:09
after dusk -> LuxAB luciferase (Vibrio fischerii)
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K743015_sequence (Version 1) |
Description
This part is designed for the expression of the LuxA and LuxB genes from Vibrio fischeri in a circadian manner, peaking a 2 hours after subjective dusk (or hour 14 of circadian time if you prefer).When transfromed into Syenchocystis PCC6803, the endogenous circadian transcriptional mechanism controls the activity of the Pta promoter making the heterodimeric LuxAB luciferase to be expressed at the specified time.
This permits for the emition of light by the luciferase reaction to be restricted to this time of the day (dependent on the addition or endogenous production of substrates)
Notes
This part was made by Gibson assembly, therefore it has no scarsSource
Pta promoter was amplified from genomic Synechocystis DNA, you can find a biobrick version of this promoter at blahblahblah.LuxAB genes were obtained from the Luxbrick
| Sequence Annotation | Location | Component / Role(s) |
| Pta BBa_K743003 promoter putative endogenous rbs LuxA v. fischeri rbs stop codon LuxB v. fischeri stop codon | 1,150 120,150 151,1212 1216,1253 1213,1216 1254,2231 2232,2234 | feature/promoter promoter ribosome_entry_site feature/rbs CDS feature/protein feature/rbs ribosome_entry_site stop_codon feature/stop feature/protein CDS feature/stop stop_codon |