Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Menuka Samaranayake
Date created: 2012-09-24 11:00:00
Date modified: 2015-05-08 01:13:13
Translational Coupling Cassette
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K762000_sequence (Version 1) |
Description
In synthetic biology, a powerful method for the production of novel metabolites is the expression of heterologous genes in Escherichia coli. A common challenge when using non-native genes in metabolic engineering is determining if they are being properly expressed. To address this issue, we have constructed a BioFusion compatible system for testing the translation of a gene of interest. This system couples the translation of the target gene to a fluorescent reporter gene. Fluorescence will only be detected when the target gene is entirely translated. This construct enables synthetic biologists to quickly determine if a gene is being expressed without the need for costly antibodies or analytical instruments (e.g. mass spectrometry).Notes
Adapted design from Mendez-Perez, et al.Source
Mendez-Perez, D., S. Gunasekaran, V. J. Orler, and B. F. Pfleger. "A Translation-coupling DNA Cassette for Monitoring Protein Translation in Escherichia Coli." Metabolic Engineering 4 (2012): 298-305.| Sequence Annotation | Location | Component / Role(s) |
| His-Tag for fused Target Gene Stop Codon for fused Target Gene RBS for Response Gene Red Florescent Protein ORF mRNA Hairpin | 1,18 19,21 24,29 38,715 2,36 | feature/tag tag feature/stop stop_codon feature/rbs ribosome_entry_site sequence_feature feature/misc feature/stem_loop stem_loop |