BBa_K766003

BBa_K766003 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K766003
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yu Zhao
Date created: 2012-09-21 11:00:00
Date modified: 2015-05-08 01:13:14

C6HSL sensor and reporter



Types
DnaRegion

Roles
Reporter

engineered_region

Sequences BBa_K766003_sequence (Version 1)

Description

We build this part to work as a receiver. C6HSL from the neighbor cell can diffuse across the membrane and activate the transcription from Plux promoter. There is something wrong with original Part:BBa_J09855. So we rebuild the reporter part which can be activated by AHL and add the reporter part RFP which is easily detectable. The expression of it is very clear.

Notes

We want to receive that signal from neighbor cell and report to us.

Source

There is something wrong with original Part:BBa_J09855. So we rebuild the reporter part which can be activated by AHL and add the reporter part RFP which is easily detectable.

Sequence Annotation Location Component / Role(s)
RBS
Plac promoter
LuxR
T1 terminator
TE terminator
Plux promoter
RBS
RFP
T1 terminator
46,57
1,37
64,819
853,932
940,981
990,1044
1060,1071
1078,1785
1792,1871
ribosome_entry_site feature/rbs
promoter feature/promoter
CDS feature/cds
feature/stop stop_codon
stop_codon feature/stop
feature/promoter promoter
feature/rbs ribosome_entry_site
CDS feature/cds
feature/stop stop_codon
igem#experience
Works
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K766003/1