pT3_xyIE

BBa_K784004 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K784004

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Noa Katz

Date created: 2012-09-12 11:00:00

Date modified: 2015-05-08 01:13:21

pT3_RBS_xyIE reporter gene

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K784004_sequence (Version 1)

Description

This part's purpose is to testify to the presence of the T3 RNA Polymerase by the xyIE reporter gene from iGEM2006_Edinburgh. It consists of an RNA T3 promoter, an RBS, a reporter gene- xyIE, and a T7 terminator. The assay for xyIE is very simple, refer to the xyIE basic part for instructions.


The xyIE+RBS part is between XhoI and XmaI restriction sites, and therefore can be excised by these enzymes.

Notes

No particular considerations were required.

Source

The RBS and xyIE genes are from the parts registry, made by iGEM2006_Edinburgh.
The promoter and terminator are from:

http://nar.oxfordjournals.org/content/early/2012/06/28/nar.gks597.full

Components

Access	Instance	Definition
public	BBa_K784003	BBa_K784003
public	BBa_J33204	BBa_J33204
public	BBa_K784002	BBa_K784002

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K784003	1,23	BBa_K784003
BBa_J33204	32,989	BBa_J33204
BBa_K784002	998,1044	BBa_K784002

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K784004/1

Member of these Collections

• iGEM Parts Registry