Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ilya Vainberg Slutskin
Date created: 2012-09-12 11:00:00
Date modified: 2015-05-08 01:13:21
Theophylline riboswitch 12.1
| Types | DnaRegion |
| Roles | mature_transcript_region RNA |
| Sequences | BBa_K784005_sequence (Version 1) |
Description
A riboswitch which is comprised of an aptamer which recognizes the ligand, theophylline. The riboswitch also contains an RBS. In the absence of theophylline, there is extensive base pairing in the 5' untranslated region (UTR) which blocks the RBS. This base pairing prevents translation of a gene downstream to the riboswitch. In the presence of theophylline, the transcript adopts a secondary structure in which the RBS is exposed, allowing translation to occur.Notes
The part must be directly fused to the starting codon of the downstream coding region.Source
The part was obtained from the Gallivan lab. The description of the part was found in the following paper:Lynch Sean A., Gallivan Justin P. 2009. A flow cytometry-based screen for synthetic riboswitches. Nucleic Acids Research 37(1): 184-192.
| Sequence Annotation | Location | Component / Role(s) |
| Additional 5' UTR Theophylline aptamer RBS | 1,17 18,55 56,74 | feature/misc sequence_feature sequence_feature feature/misc ribosome_entry_site feature/rbs |