pT3+phoA

BBa_K784029 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K784029
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lior Levy
Date created: 2012-09-16 11:00:00
Date modified: 2015-05-08 01:13:22

pT3+RBS+Alkaline Phosphatase (pHO)+Terminator



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K784029_sequence (Version 1)

Description

This part consists of T3 RNA polymerase induced promoter followed by an RBS and the ‎Alkaline Phosphatase reporter gene (pHOA) from Citrobacter.‎
The part's purpose is to monitor the translation of the T3 RNA polymerase with the Alkaline ‎Phosphatase gene which can be quantified with the PNP (para-nitrophenol phosphate) hydrolysis assay that has been ‎made by Arkin Lab group in 2006.‎
The Alkaline Phosphatase + RBS part is between XmaI and XhoI restriction sites and ‎therefore can be excised out by these enzymes.

Notes

we used XhoI and XmaI restriction sites for the ligation because these sites are not anywhere in the sequence.

Source

The promoter and terminator are from:Temme Karsten, et al. 2012. Modular control of multiple pathways using engineered orthogonal T7 polymerases. Nucleic Acids Research (advance access): 1-9.
The RBS is from the parts registry,BBa_B0064 made by Endi Lab (2008)
The pHOA gene is from the parts registry,BBa_J61032 made by Arkin Lab (2006)

igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K784029/1