Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Noa Katz
Date created: 2012-09-16 11:00:00
Date modified: 2015-05-08 01:13:22
pN4_RBS_xyIE reporter gene
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K784032_sequence (Version 1) |
Description
This part's purpose is to testify to the presence of the N4 RNA Polymerase by the xyIE reporter gene from iGEM2006_Edinburgh. It consists of an RNA N4 promoter, an RBS, a reporter gene- xyIE, and a T7 terminator. The assay for xyIE is very simple, refer to the xyIE basic part for instructions- BBa_J33204.The xyIE+RBS part is between XhoI and XmaI restriction sites, therefore can be excised by these enzymes.
Notes
We had to make sure that the part doesn't have xhoI and xmaI restriction sites.Source
The RBS and xyIE genes are from the parts registry, made by iGEM2006_Edinburgh.The promoter and terminator are from:Temme Karsten, et al. 2012. Modular control of multiple pathways using engineered orthogonal T7 polymerases. Nucleic Acids Research (advance access): 1-9. The RBS is from the parts registry,BBa_B0064 made by Endi Lab (2008) The pHOA gene is from the parts registry,BBa_J61032 made by Arkin Lab (2006)
| Access | Instance | Definition |
| public public public | BBa_K784031 BBa_K784024 BBa_K784002 | BBa_K784031 BBa_K784024 BBa_K784002 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K784031 BBa_K784024 BBa_K784002 | 1,976 985,1007 1016,1062 | BBa_K784031 BBa_K784024 BBa_K784002 |