Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Leung Wai Tak
Date created: 2012-09-20 11:00:00
Date modified: 2015-05-08 01:13:22
Sensory rhodopsin II (SRII) with HtrII & Tsr, sensitive to blue-green light
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K786001_sequence (Version 1) |
Description
Sensory rhodopsin II fusion with HtrII & Tsr, which is sensitive to blue-green light, with sensing spectra covering from 400-500 nm.We used it for sensing blur-green light to achieve phototaxis and switch for downstream gene expression.
Notes
Pfam (version 26.0) was used to predict the domain of fusion protein.Linker was refereed and modified from previous research study of SRII fusion with HtrII.
Source
From genomic sequence of bacterial N. pharaonis (DSM 2160) and E.coli K12.| Sequence Annotation | Location | Component / Role(s) |
| Constitutive promoter BBa_J23100 RBS 1.0 HindIII for N-terminal fusin Sensory Rhodopsin II linker BamHI for C-terminal fusion HtrII Tsr B0015 | 1,42 43,55 62,67 68,784 785,811 785,790 812,1210 1210,2070 2105,2233 | feature/promoter promoter feature/rbs ribosome_entry_site sequence_feature feature/misc CDS feature/protein feature/protein CDS sequence_feature feature/misc feature/protein CDS feature/protein CDS engineered_region feature/BioBrick |