BBa_K801003

BBa_K801003 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K801003
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Georg Sch??tzinger
Date created: 2012-09-20 11:00:00
Date modified: 2015-05-08 01:13:24

pTUM103 yeast shuttle vector with pADH1 promoter



Types
DnaRegion

Roles
plasmid_vector

Plasmid_Backbone

Sequences BBa_K801003_sequence (Version 1)

Description

This part corresponds to pTUM100 (BBa_K801000). The constitutive pADH1 promoter has been cloned in front of the MCS (RFC25 prefix and suffix).

Notes

All forbidden restriction sites have been removed from the plasmid backbone, making it RFC25 compatible .

Source

Based on invitrogen's pYES2 plasmid. For more information see the part information of pTUM100 (BBa_K801000).

Sequence Annotation Location Component / Role(s)
CYC1-Terminator
URA3
AmpR
ADH1-Promoter
T7-Promoter
2 micron (??) origin
pUC-origin
810,1057
2936,4043
2058,2917
58,775
4,23
4047,5518
1240,1913
feature/stop stop_codon
CDS feature/cds
CDS feature/cds
feature/promoter promoter
primer_binding_site feature/primer_binding
feature/dna sequence_feature
feature/misc sequence_feature
igem#experience
Works
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K801003/1