GFP-AID
BBa_K812110 Version 1 Component
This part has been discontinued.
Please consider using BBa_K812010 as a replacement.
Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Pierre Parutto
Date created: 2012-09-19 11:00:00
Date modified: 2015-05-08 01:13:27
GFP fusion with the ubuquitinase E3 OsTirI recoginition domain for PSC2+ plasmid
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K812110_sequence (Version 1) |
Description
This part is a super folded GFP fused to an AID tag for its recignition by the ubiquitinase E3 that induces its degradation in the presence of auxin in the eukaryote cell.This part is a part of a device pattented by Kanemaki Masato, Kakimoto Tatsuo, Nishimura Kohei, Takisawa Haruhiko and Fukagawa Tatsuo for yeast and mammalian cells use (http://www.freepatentsonline.com/y2012/0115232.html). However the pattent does not cover the use for oviparian such as frogs and chicken.
The GFP-AID contains 2 NLS domains that relocate the protein in the nucleus of the cells for a better signal concentration.
This part can be used in an oviparian plasmid such as pSC2+ (see K812000)
Notes
This part has been biobricked using PCR method and cloned in pSB1C3.Source
The source plasmid was provided in K. Nishimura, T. Fukagawa, H. Takisawa, T. Kakimoto, and M. Kane-maki. An auxin-based degron system for the rapid depletion of proteins in nonplant cells. Nature methods, 6(12) :917???922, 2009.