BBa_K812133

BBa_K812133 Version 1

Component

This part has been discontinued.

Please consider using BBa_K812031 as a replacement.

Source:
http://parts.igem.org/Part:BBa_K812133
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Anna Mlynarczyk
Date created: 2012-09-19 11:00:00
Date modified: 2015-05-08 01:13:28

sfGFP with kozak sequence for expression in Xenopus



    • Details

    Types
    DnaRegion

    Roles
    Reporter

    engineered_region

    Sequences BBa_K812133_sequence (Version 1)

    Description

    Super folded GFP fused with a kozak sequence to enhance its expression in eukaryotic cells. To be used with an eukaryotic expression system such as pSC2+ (K812000) and related parts. Ready for immediate use in Xenopus.

    Notes

    Kozak is included, as it cannot be added by biobrick. This part is ready for direct injection into a fertilised Xenopus tropicalis egg.

    Source

    Original sequence from A. victoria mutated to emit green light

    Sequence Annotation Location Component / Role(s)
    kozak
    cds
    		1,6
    7,713
    		sequence_feature feature/misc
    feature/cds CDS

    igem#experience
    Works
     
    igem#sampleStatus
    Discontinued
    igem#status
    Deleted
     
    isReplacedBy
    BBa_K812031/1
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K812133/1
     

    Attachments

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