BBa_K819000

BBa_K819000 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_K819000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Haoqian Zhang

Date created: 2012-08-30 11:00:00

Date modified: 2015-05-08 01:13:29

Luminescence-responsive Promoter+GFP(BBa_E0840)

Details

• Types: DnaRegion
• Roles: Reporter; engineered_region
• Sequences: BBa_K819000_sequence (Version 1)

Description

The luminescence-responsive promoter was engineered from promoter ColE7, during which the LexA binding site was mutated to guarantee that the endogenous LexA will not disturb the function of this promoter. This mutated LexA binding site within luminescence-responsive promoter can be recognized by corresponding mutated LexA DNA binding domain.

Notes

The LexA binding site was mutated to guarantee that the endogenous LexA will not disturb the function of this promoter. This mutated LexA binding site within luminescence-responsive promoter can be recognized by corresponding mutated LexA DNA binding domain.

Source

ColE7 promoter, ColE plasmid

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
LexA408 DNA binding domain	1,4	feature/operator, operator

Other Properties

• igem#experience: Works
• igem#sampleStatus: Discontinued
• igem#status: Deleted
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K819000/1

Member of these Collections

• //function/reporter/light
• iGEM Parts Registry