Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: ZHANG Zidong
Date created: 2012-09-02 11:00:00
Date modified: 2015-05-08 01:13:29
Lux Operon under T7 promoter
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K819008_sequence (Version 1) |
Description
luxABCDE gene ligated to the rear of T7 promoter. When transformed into BL21(DE3) E.coli strain, add different amount IPTG to reach different final concentration of IPTG in the medium can induce different level of Lux ligt emission. No substrate is needed.Notes
luxABCDE gene ligated to the rear of T7 promoter. The original Ara and P-bad were replaced with T7 promoter, which enables its usage in our project.Source
T7 promoter and LuxCDABE with their RBS are from Partsregistry.| Sequence Annotation | Location | Component / Role(s) |
| BBa_K819008 LuxCDABEG with corresponding six RBS(from K325909) | 1,46 53,6480 | feature/promoter promoter feature/misc sequence_feature |