pSBBs4S

BBa_K823022 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K823022

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jara Radeck

Date created: 2012-08-15 11:00:00

Date modified: 2015-05-08 01:13:30

pSB_Bs4S: Empty backbone for integration into Bacillus subtilis thrC locus

Details

• Types: DnaRegion
• Roles: Plasmid_Backbone; plasmid_vector
• Sequences: BBa_K823022_sequence (Version 1)

Description

This part is an empty backbone vector for the usage in Bacillus subtilis. It integrates in the thrC locus and can be selected with Spectinomycin cassette. It has a ampicillin resistance for cloning in E.coli. It originates from the Bacillus vector pDG1731.

Notes

This plasmid can be used as empty backbone vectors like pSB1C3 but is able to integrate in B.subtilis. The clonging site also includes a rfp for selection.

Source

B.subtilis vector pDG1731

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
spc	211,885	feature/cds, CDS
'thrC	1028,1456	sequence_feature, feature/misc
thrB	1453,1863	sequence_feature, feature/misc
ori approx.	1942,2556	sequence_feature, feature/misc
bla	2716,3576	CDS, feature/cds
hom	3731,4033	feature/cds, CDS
thrC'	4033,4542	feature/misc, sequence_feature

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K823022/1

Member of these Collections

• //chassis/multihost
• //chassis/prokaryote/bsubtilis
• //chassis/prokaryote/ecoli
• //plasmid/chromosomalintegration
• //plasmidbackbone/operation
• iGEM Parts Registry
• igem_plasmid_vectors