pSBBs0K

BBa_K823026 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K823026

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jara Radeck

Date created: 2012-08-20 11:00:00

Date modified: 2015-05-08 01:13:30

pSB_Bs0K-P_spac (replicative Bacillus subtilis expression vector
IPTG inducible

Details

• Types: DnaRegion
• Roles: plasmid_vector; Plasmid_Backbone
• Sequences: BBa_K823026_sequence (Version 1)

Description

This part is an expression vector for Bacillus subtilis. It is replicative both in E.coli and B.subtilis. It has a ampicillin resistance for cloning in E.coli and kanamycin resistance for selection in B. subtilis. The multiple cloning site is downstream of a Pspac promoter, that is inducible with IPTG. (Isopropyl-β-D-thiogalactopyranosid).

This BioBrick is part of the LMU 2012 igem project Bacillus BioBrickBOX http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks

Notes

We removed any forbidden restriction sites by site-directed mutagenesis PCR. The clonging site also includes a rfp for selection for cloning in E.coli. It can be used as an inducible expression vector in B. subtilis.

Source

This part is an igem compatible version of the Bacillus expression vector pDG148

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
lacI	271,1353	CDS, feature/cds
bla	2609,3469	feature/cds, CDS
repU	4093,5097	feature/misc, sequence_feature
kan	5257,6027	feature/cds, CDS
ble	6250,6648	CDS, feature/cds
lacY	6906,7871	CDS, feature/cds
Pspac	8193,8258	promoter, feature/promoter

Other Properties

• igem#experience: Issues
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K823026/1

Member of these Collections

• //chassis/multihost
• //chassis/prokaryote/ecoli
• //chassis/prokaryote/subtilis
• //plasmidbackbone/expression/inducible
• iGEM Parts Registry
• igem_plasmid_vectors