Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Julianne Grose
Date created: 2012-10-01 11:00:00
Date modified: 2015-05-08 01:13:32
Lactate sensor derived from the MglB protein from E. coli
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K833012_sequence (Version 1) |
Description
This part is a lactate sensor derived from site directed mutagenesis of the E. coli MglB protein.Notes
Designing primers to incorporate a large number of site-directed mutationsSource
This part was derived from site directed mutagenesis of the E. coli MglB protein.| Sequence Annotation | Location | Component / Role(s) |
| Y33K (Y10K in truncated version from Looger et al.) Y37K (D14K in truncated version from Looger et al.) N114K, K115L (N91K, K92L in truncated version from Looger et al.) H175M, D177H (H152M, D154H in truncated version from Looger et al.) W206K (W183K in trucnated version from Looger et al.) D259A ( D236A in truncated version from Looger et al.) N279D (N256D in truncated version from Looger et al.) H175M (H152M in truncated version from Looger et al.) D178H (D154H in truncated version from Looger et al.) | 97,99 109,111 342,345 541,543 616,618 776,777 835,837 523,525 529,529 | sequence_alteration feature/mutation feature/mutation sequence_alteration sequence_alteration feature/mutation feature/mutation sequence_alteration feature/mutation sequence_alteration feature/mutation sequence_alteration feature/mutation sequence_alteration feature/mutation sequence_alteration sequence_alteration feature/mutation |