LipA

BBa_K836000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K836000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Ricardo Alvarado

Date created: 2012-09-17 11:00:00

Date modified: 2015-05-08 01:13:32

lipA from B. cepacia (codon usage optimized for E. coli)

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K836000_sequence (Version 1)

Description

This biobrick codifies for the protein lipA from Burkholderia cepacia (Pseudomonas cepacia) which catalyzes the hydrolysis of triglycerides (Triacylglycerol + H2O = diacylglycerol + a carboxylate). In the presence of certain alcohols, it catalyzes the transesterification of TGAs to produce acyl esters and glycerol.

It has only one PTM (Post-Translational Modification) which consists in a sulfide bond between 234 and 314 aa. Calcium serves as a cofactor for this enzyme (1 ion per subunit).

Notes

The sequence of a transcriptional terminator (biobrick BBa_B1006) was set at the end of the coding sequence. Also, to avoid RBS-CDS problems, the CDS prefix and suffix mentioned in this link (http://partsregistry.org/Assembly:RBS-CDS_issues#Standard_Assembly_and_the_RBS) were used.

Source

The amino sequence of this part was taken from Uniprot (lipA accesion P22088; http://www.uniprot.org/uniprot/P22088) and then codon optimization (avoiding restriction sites for the standard assembly method) was made using the free software GeneDesigner.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
lipA	1,1098	CDS, feature/cds

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K836000/1

Member of these Collections

• //cds/biosynthesis
• //cds/enzyme
• iGEM Parts Registry