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BBa_K836005

BBa_K836005 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K836005

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Ricardo Alvarado

Date created: 2012-09-19 11:00:00

Date modified: 2015-05-08 01:13:32

Lysozyme from Rhodococcus phage RER2 (codon usage optimized for R, opacus)

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Details

Types	DnaRegion
Roles	engineered_region Translational_Unit
Sequences	BBa_K836005_sequence (Version 1)

Description

Protein involved in the peptidoglycan degradation of the bacterial cell wall. A RBS for Gram positive bacteria (SpoVG) was added at the beginning.

Notes

A spacer was put between the RBS and the start codon which corresponds to the scar between a normal suffix and the prefix used for CDS to avoid RBS-CDS problems

Source

The amino sequence was obtained from Uniprot (http://www.uniprot.org/uniprot/G9FHW6) and then it was optimized for R. opacus (avoiding standard restriction sites).

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
SpoVG Scar Lysozyme	1,12 13,18 19,1347	feature/rbs ribosome_entry_site sequence_feature feature/misc CDS feature/cds