BBa_K842000

BBa_K842000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K842000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Sean P. Curran, Percy Genyk, Ellen Park, Rebecca, Gao, Stephan Genyk, Megan Bernstein, Rachel Kohan, Eric Siryj, Luke Quinto

Date created: 2012-10-01 11:00:00

Date modified: 2015-05-08 01:13:33

flhB

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K842000_sequence (Version 1)

Description

Class 2 flagella operon. When transcribed, flhB is one of three flagella genes responsible for the generation of proteins that export flagellin to the flagella apparatus. The flagellin is then directly used in the synthesis of the structures??? basal body. Additionally, flhB directly transcribes the operon flhBAE.

Cloning and Uses
flhB was use generated via PCR from the E. coli strain DH5α and cloned into the vector pSB1C3. It is directly downstream of the class 1 flagella operons flhD and flhC. It also works in conjunction with fliL and fliH to make up the flagellin export system. This part can be used either to generate a flagella in an E. coli strain in which they are not already present, or to promote further formation of pre-existing flagella.

Used for reconstitution of flagella apparatus in E. coli B strains.

Notes

None

Source

Flagellar biosynthetic protein flhB ??? Escherichia coli (strain K12). (n.d.). UniProt. Retrieved October 1, 2012, from http://www.uniprot.org/uniprot/P76299

flhB:Quickview ??? EcoliWiki. (n.d.). EcoliWiki. Retrieved October 1, 2012, from http://ecoliwiki.net/colipedia/index.php/flhB:Quickview

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
flhB	1,1149	engineered_region, feature/BioBrick

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K842000/1

Member of these Collections

• iGEM Parts Registry