Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sean P. Curran, Percy Genyk, Ellen Park, Rebecca, Gao, Stephan Genyk, Megan Bernstein, Rachel Kohan, Eric Siryj, Luke Quinto
Date created: 2012-10-01 11:00:00
Date modified: 2015-05-08 01:13:33
fliA
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K842003_sequence (Version 1) |
Description
fliA is an alternate sigma factor for the class 3 flagella operons. When transcribed, it forms a component of RNA polymerase sigma 28.Used for reconstitution of flagella apparatus in E. coli B strains.
Cloning and Uses
This part was generated via PCR from the E. coli strain DH5α and cloned into the vector pSB1C3. It is to be used in conjunction with the entire flagella synthesis genetic pathway to reproduce a complete flagella apparatus.
Notes
noneSource
fliA:Quickview ??? EcoliWiki. (n.d.). EcoliWiki. Retrieved October 1, 2012, from http://ecoliwiki.net/colipedia/index.php/fliAIde, N., & Kutsukake, K. (n.d.). Identification of a novel Escherichia coli gene whose e??? [Gene. 1997] ??? PubMed ??? NCBI. National Center for Biotechnology Information. Retrieved October 1, 2012, from http://www.ncbi.nlm.nih.gov/pubmed/9358034
Claret, L., Miquel, S., Vieille, N., Ryjenkov, D., Gomelsky, M., & Darfeuille-Michaud, A. A. (2007). The Flagellar Sigma Factor FliA Regulates Adhesion and Invasion of Crohn Disease-associated Escherichia coli via a Cyclic Dimeric GMP-dependent Pathway. The Journal Of Biological Chemistry, 282(46), 33275-33283. Retrieved October 1, 2012, from http://www.jbc.org/content/282/46/33275.full.pdf