BBa_K842005

BBa_K842005 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K842005

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Sean P. Curran, Percy Genyk, Ellen Park, Rebecca, Gao, Stephan Genyk, Megan Bernstein, Rachel Kohan, Eric Siryj, Luke Quinto

Date created: 2012-10-01 11:00:00

Date modified: 2015-05-08 01:13:33

fliH

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K842005_sequence (Version 1)

Description

FliH functions as part of the flagella specific export system and is necessary for flageller growth and assembly. It is believed to be part of a two-component system consisting of fliH and fliL that hydrolyses ATP until construction of the flagella apparatus is completed.

Used for reconstitution of flagella apparatus in E. coli B strains.

Cloning and Uses
This part was generated via PCR from the E. coli strain DH5α and cloned into the vector pSB1C3. It is to be used in conjunction with the entire flagella synthesis genetic pathway to reproduce a complete flagella apparatus.

Notes

none

Source

Minamino, T., & MacNab, R. (n.d.). FliH, a soluble component of the type III flag??? [Mol Microbiol. 2000] ??? PubMed ??? NCBI. National Center for Biotechnology Information. Retrieved October 1, 2012, from http://www.ncbi.nlm.nih.gov/pubmed/10998179

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
fliH	1,687	CDS, feature/cds

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• rdf-schema#seeAlso: P31068
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K842005/1

Member of these Collections

• iGEM Parts Registry