BBa_K842006

BBa_K842006 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K842006
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sean P. Curran, Percy Genyk, Ellen Park, Rebecca, Gao, Stephan Genyk, Megan Bernstein, Rachel Kohan, Eric Siryj, Luke Quinto
Date created: 2012-10-01 11:00:00
Date modified: 2015-05-08 01:13:33

cheA



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K842006_sequence (Version 1)

Description

CheA is a regulatory protein that becomes activated when the methyl-accepting chemotaxis protein (MCP) is not bound to any attractant. The activated cheA self-phosphorylates itself by hydrolyzing ATP in order to gain a phosphate group. CheA then donates its phosphate group to activate cheB or cheA.

Used for researcher control of signaling mechanisms that influence flagella function.

Cloning and Uses
This part was generated via PCR from the E. coli strain DH5α and cloned into the vector pSB1C3. It is to be used with other chemotaxis genes to form a complete regulatory pathway that controls the response system in MCP and the direction of rotation in flagella.

Notes

none

Source

Chemotaxis protein CheA ??? Escherichia coli (strain K12). (n.d.). UniProt. Retrieved October 1, 2012, from http://www.uniprot.org/uniprot/P07363

Sequence Annotation Location Component / Role(s)
CheA
1,1965
CDS feature/cds
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K842006/1