BBa_K842012

BBa_K842012 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K842012
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sean P. Curran, Percy Genyk, Ellen Park, Rebecca, Gao, Stephan Genyk, Megan Bernstein, Rachel Kohan, Eric Siryj, Luke Quinto
Date created: 2012-10-01 11:00:00
Date modified: 2015-05-08 01:13:33

motB



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K842012_sequence (Version 1)

Description

motB is a structural protein for the stationary element of the flagella motor. The protein works in conjunction with motA to form this structure, which then in turn controls the flagella???s rotation. It is also believed that motB is responsible for forming the structure that connects the flagella motor to the cell wall.

Used for reconstitution of flagella apparatus in E. coli B strains.

Cloning and Uses
This part was generated via PCR from the E. coli strain DH5α and cloned into the vector pSB1C3. The protein works in conjunction with motA to form the stationary base of the flagella motor, which then in turn controls the flagella???s rotation. This part can be used in the complete recreation of an E. coli???s genetic flageller pathway.

Notes

none

Source

Motility protein B ??? Escherichia coli (strain K12). (n.d.). UniProt. Retrieved October 1, 2012, from http://www.uniprot.org/uniprot/P0AF06

Sequence Annotation Location Component / Role(s)
motB
1,927
feature/cds CDS
igem#experience
None
 
igem#partStatus
Released HQ 2013
igem#sampleStatus
In stock
igem#status
Available
 
rdf-schema#seeAlso
P0AF06
 
rdf-schema#seeAlso
P0AF07
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K842012/1