BBa_K862000

BBa_K862000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K862000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Charlotte Bunne, Mariam Harmouche, Stefan Holderbach, Anna Huhn and Jakob Kreft (in alphabetical order)
Date created: 2012-06-07 11:00:00
Date modified: 2015-05-08 01:13:36

precA-LacZ-doubleTerminator



Types
DnaRegion

Roles
engineered_region

Measurement

Sequences BBa_K862000_sequence (Version 1)

Description

This is a part for the precise quantification of UV-radiation or radioactive radiation in E. coli (rec+) strains, i.e. BL21(DE3). It consits of a recA Promoter (part BBa_J22106) fused to a LacZ reporter cloned in front of a double terminator (part BBa_K173004).

Notes

The part was first constructed in plasmid backbone pSB1AK3 and then subcloned into pSB1C3 for submission to the registry.

Source

The part was constructed by standard cloning from parts BBa_J22106 (pRecA) and part BBa_K173004 (LacZ reporter).

igem#experience
Works
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K862000/1