BBa_K864005

BBa_K864005 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K864005
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Erik Lundin
Date created: 2012-09-22 11:00:00
Date modified: 2015-05-08 01:13:37

Low copy BioBrick standard vector



Types
DnaRegion

Roles
Plasmid_Backbone

plasmid_vector

Sequences BBa_K864005_sequence (Version 1)

Description

pSB4A15 is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.

The resistance cassette is flanked by FRT sites (BBa_J61020) which allows for subsequent removal
of the resistance cassette from the chromosome by the FLP recombinase. The pSB8x15 series are especially suitable for carrying the FLP recombinase, since they can be removed by incubation at 42??C.

Notes

The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is.
The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone.
The pSC101 origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.

Source

Based on pSB3T5

Sequence Annotation Location Component / Role(s)
Lambda Red reverse primer
TonB terminator
VR
rrnC terminator
pSC101 ori BBa_K864050
Lambda Red forward primer
Lux terminator
18,38
47,115
116,135
136,175
190,2405
2433,2450
2454,2499
primer_binding_site feature/primer_binding
feature/stem_loop stem_loop
feature/primer_binding primer_binding_site
stem_loop feature/stem_loop
sequence_feature feature/misc
feature/primer_binding primer_binding_site
feature/stem_loop stem_loop
igem#experience
Works
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K864005/1